Composite

Part:BBa_K5236022

Designed by: Hening Guo   Group: iGEM24_BASIS-China   (2024-10-01)


pET28a-pelB-BhrPETase(M57L)-6xHis

This composite part codes for the mutated BhrPETase M57L and expresses the enzyme in the extracellular space of chassis.

T7 promoter: used to regulate gene expression of recombinant proteins.

Lac operator: used to control gene expression by lactose.

RBS: the location to bind ribosome.

BhrPETase M57L: This sequence encodes for a mutated BhrPETase M57L and is derived from Escherichia coli.

pelB: the sequence codes for signal peptide which helps PETase exit the cell membrane.

6xHis: a tag for the purification of protein which focus in the production of recombinant proteins.

T7 terminator: terminates transcription.


Fig.1 Constructed Plasmid

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 308
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 218
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 912


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Parameters
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